An analytical affinity chromatography assay has been developed for the investigation of the dissociation of antigen-antibody complexes. Albumin-coupled Sepharose 4B and anti-albumin has been used as a model system. At extremely low or high pH, in the presence of highly concentrated chaotropic ions at pH 7 or by elution with 100% ethylene glycol after pretreating with high pH buffer, most of the bondings could be Air Jordan Retro 3
ruptured. The latter two-step desorption procedure provides recovery of intact antibody with high yield. The technique was also utilized for the preparation of antibody against human growth hormone.Antigen-antibody complexAffinity chromatographyMild desorption
Steel pipe lined Fe-Al intermetallic-TiB2-Al2O3 composite were prepared by centrifugal-self-propagating high temperature synthesis (centrifugal-SHS) process from FeTiO3, B2O3, Fe2O3 and Al as the raw materials. The standard Gibbs energy minimization Cheap Air Jordan Shoes Online method was used to calculate the equilibrium compositions of the reacting species. The effect of molar ratio of precursors and rotating speed on result product were investigated. It was shown that the composites layer had less porosity as higher rotating speed was applied. The phase composite and morphology were characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM) couple with Energy dispersive Air Jordan Women
Xray (EDX), respectively.