In vitro compartmentalisation in an emulsion was used to physically link proteins to the DNA that encodes them via microbeads. These microbeads can be selected for catalysis, or, as demonstrated here, for binding. Genes encoding a peptide containing an epitope (haemagglutinin) were enriched to near purity from a 106-fold excess Air Jordan 6 Retro
of genes encoding a different peptide by two rounds of selection using flow cytometry, indicating ∼1000-fold enrichment per round. Single beads can be isolated using flow sorting and the single gene on the bead amplified by polymerase chain reaction. Hence, the entire process can be performed completely in vitro.
Cephalopods Air Jordan 4 Toro Bravo are sensitive to the linear polarization characteristics of light. To examine if this polarization sensitivity plays a role in the predatory behavior of cuttlefish, we examined the preference of Sepia officinalis when presented with fish whose polarization reflection was greatly reduced versus fish whose polarization reflection was not affected. Cuttlefish preyed preferably on fish with normal polarization reflection over fish that did not reflect linearly polarized light (n=24, χ2=17.3, P<0.0001), implying that polarization sensitivity is used during predation. We suggest that polarization vision is used to break the
countershading camouflage of light-reflecting silvery fish.