A quantitative tissue culture assay for bovine papilloma virus (BPV) has been developed. Extracts of bovine papillomas which contained either BPV-1 or BPV-2 induced distinct foci in two mouse cell lines (NIH 3T3 and C127). Focus formation followed single-hit kinetics, suggesting that a single particle was required for focus induction. Preincubation of extracts with rabbit anti-BPV-1 serum neutralized their infectivity. The morphologically transformed cells contained BPV DNA sequences, grew in low serum and in soft agar, and were tumorigenic for nude mice.
Fourteen non-hypoplastic congenitally deformed ears were treated with a simple splint with Air Jordan 5 Retro satisfactory results in all but one. These deformities can be treated non-surgically in the early neonatal period, effectively, Air Jordan Retro
without anaesthetic, and with minimal cost. We confirm other reports that better results are obtained and a shorter duration of splintage is needed if treatment is started early in the neonatal period. Our Jordan Retro 5
neonatal paediatric colleagues and midwives should be encouraged to manage these deformities in this way, and the need for surgical correction may be largely avoided in the future.